Probing higher order multimers of pyruvate kinase with charge detection mass spectrometry
نویسندگان
چکیده
Pyruvate kinase multimers have been investigated by charge detection mass spectrometry (CDMS). In CDMS, the m/z and z are simultaneously measured for each ion, so the mass is determined directly. The measurements were made using a modified cone trap that incorporates an image charge detector with a cryogenically cooled preamplifier. With non-denaturing solution conditions, the tetrameric form of pyruvate kinase is observed along with aggregates of the tetramer. The time-of-flight m/z spectrum shows
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